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Location: Worcester, MA
Business Unit: UMass Med School
Department: School - Neurology - W832500
Job Type: Full-Time
Salary Grade: 42
Num. Openings: 1
Post Date: Feb. 17, 2021
GENERAL SUMMARY OF POSITION:
Dr. Yingleong (Rigel) Chan's laboratory seeks a highly motivated individual with substantial biological mammalian cell culture expertise to perform in-vitro experimentation with human cells. The applicant will be responsible for a wide array of biological assays, including the generation and culture of human-induced pluripotent stem cells (iPSCs), differentiating them into various adult tissue types (microglia, astrocytes, neural progenitors, cerebral organoids, etc.) and performing in-vitro phenotypic assays on them, including viral infection as well as determining various immune phenotypes. Other phenotypes also include cell-death due to exposure to certain drugs or chemicals. Phenotyping is performed mainly using flow cytometry as well as cell sorting using FACS. Depending on the level of expertise and the research interest of the individual, the position allows for developing computational and statistical methods in the context of ongoing projects.
Under the direction of the Principal Investigator or designee, the Research Associate I performs a variety of complex research experiments.
- Perform complex laboratory experiments
- Conduct in-depth analysis of research experiments
- Interpret data, form conclusions, and decide on and plan the next experiments to be done with a high degree of independence
- Write standard operating procedures, schedule work, analyze data, maintain accurate records, and write reports
- Compile and analyze data. Perform computations using image analysis software, spreadsheets, graphing, and curve fitting software
- May modify protocols and be responsible for a single, independent research project
- May assist with budget and/or operational aspects of grant and contract proposals
- Assist in writing the text of scientific publications and grants. Review literature
- Train new laboratory personnel, students, and collaborators
- Comply with all safety and infection control standards
- Perform other duties as required.
- Bachelor’s degree in the Biological Sciences, or equivalent experience
- 3 years of relevant research experience
- Knowledge of Microsoft Office products
- Strong ability to analyze complex visual data in a quantitative and objective manner
- Judgment and action skills required to solve commonly encountered problems
- Experience in human cell line culture including culture of induced pluripotent stem cells (iPSCs).
- Experience in iPSCs to adult tissue type differentiation.
- Experience in viral infection of human cells.
- Experience in cell staining and flow cytometry assays.
- Experience in performing experiments required for next-generation sequencing. Genomic DNA extraction, DNA library prep, etc
Under the direction of the Principal Investigator, or designee
May functionally supervise aides, technicians, and associates
ENVIRONMENTAL WORKING CONDITIONS:
Wet chemistry laboratory, and may require appropriate contact with biohazards, radionuclides, toxins, animals, and human specimens.
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