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Category: Research
Location: Worcester, MA
Shift: Day
Exempt/Non-Exempt: Exempt
Business Unit: UMass Chan Medical School
Department: School - Molecular Cell & Cancer Biology - W495000
Job Type: Full-Time
Salary Grade: 40
Union Code: Non Union Position -W60- Non Unit Professional
Num. Openings: 1
Post Date: Dec. 5, 2018
GENERAL SUMMARY OF POSITION:
Under the direction of the Principal Investigator or Designee, the Research Associate performs a variety of complex research experiments.
Laboratory uses CRISPR/Cas9 tools for the development of gene therapy reagents.
Additional Major Responsibilities:
- Participate in the construction and protein purification of Cas9 and Cpf1 nuclease variants with increased precision, and assays for their activity in mammalian cells.
- Maintain the laboratory database and frozen archive of plasmids and bacterial strains.
- Prepare competent bacterial cells for transformation and media reagents.
- Ordering standard supplies for the laboratory.
MAJOR RESPONSIBILITIES:
- Conducts in-depth analysis of research experiments
- Performs complex laboratory experiments
- Interprets data, forms conclusion, and decides on and plans next experiments to be done with a high degree of independence
- Compiles and analyzes data and performs computations using image analysis software, spreadsheets, graphing, and curve fitting software
- May modify protocols and be responsible for a single, independent research project
- Assists in writing the text of scientific publications and grants. Reviews literature
- Trains new laboratory personnel, students, and collaborators
- Maintains laboratory supplies and equipment
- Complies with all safety and infection control standards
- Perform other duties as required.
REQUIRED QUALIFICATIONS:
- Bachelor’s degree in Biological Sciences, or equivalent experience
- 1 year relevant research experience
- Knowledge of Microsoft Office products
- Strong ability to analyze complex visual data in a quantitative and objective manner
- Judgment and action skills required to solve commonly encountered problems
PREFERRED QUALIFICATIONS:
- FPLC-based protein purification experience -required
- Mammalian Cell culture experience
- Basic molecular biology techniques (PCR, Restriction Enzyme mediated cloning, Gel electrophoresis, Qiagen DNA miniprep)
- Bacteria Strain preparation and handling (competent cell preparation and agar plate preparation).
SUPERVISION RECEIVED:
Under the direction of the Principal Investigator, or designee
SUPERVISION EXERCISED:
May functionally supervise Lab Technicians and Aides
ENVIRONMENTAL WORKING CONDITIONS:
Wet chemistry laboratory, and may require appropriate contact with biohazards, radionuclides, toxins, animals, and human specimens.
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